Optimase Master Mix Calculator

Number of reactions:
Reaction volume: µl
Allowance for error: %
Magnesium sulphate in buffer?

Reagent

Stock Concentrations

Desired Values

 Reaction Mix (µl)
Reaction buffer X X
Magnesium Sulphate mM mM
dNTPs (total) mM mM
Forward primer µM µM
Reverse primer µM µM
DNA template ng/µl ng
Optimase U/µl U
Water
Total reaction volume: µl

   

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