This tool will generate PCR protocols for use with Optimase, the high-fidelity polymerase product from Transgenomic. To generate a PCR protocol, enter your primers and anticipated PCR product length, and select a PCR protocol type. When you click on "Develop PCR protocol", this software will generate the appropriate PCR protocol.

Forward primer sequence:
Reverse primer sequence:
PCR product length: bp
Protocol type:

Note: In the sequence fields above, include only the template-specific portion of the primer--do not include any GC clamps or tails. For DHPLC applications, we recommend that the product length be between 150 and 700 bp in length.

Optimase and ProtocolWriter are trademarks of Transgenomic, Inc.